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3 edition of Cytostatic factor in Xenopus laevis. found in the catalog.

Cytostatic factor in Xenopus laevis.

Ruth Miriam.* Moses

Cytostatic factor in Xenopus laevis.

by Ruth Miriam.* Moses

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Published .
Written in English


The Physical Object
Pagination128 leaves
Number of Pages128
ID Numbers
Open LibraryOL19470379M

Daar I, Paules RS, Vande Woude GF () A characterization of cytostatic factor activity from Xenopus eggs and c-mos-transformed cells. J Cell Biol – PubMed CrossRef Google Scholar Xenopus egg extract is a lysate that is prepared by crushing the eggs of the African clawed frog Xenopus offers a powerful cell-free (or in vitro) system for studying various cell biological processes, including cell cycle progression, nuclear transport, DNA replication and chromosome is also called Xenopus egg cell-free system or Xenopus egg cell-free extract.

Fertilization is the fundamental process in which two gametes - sperm and oocyte - fuse to generate a zygote that will form a new multicellular organism. In most vertebrates, oocytes await fertilization while arrested at metaphase of meiosis II. This resting state can be stable for many hours and depends on a cytoplasmic activity termed cytostatic factor (CSF).   Nishiyama, T., Ohsumi, K. & Kishimoto, T. Phosphorylation of Erp1 by p90rsk is required for cytostatic factor arrest in Xenopus laevis eggs. Nature , – () CAS.

X. laevis egg extract Demembranated sperm nuclei and cytostatic factor (CSF)-arrested low-speed X. laevis egg extracts were prepared as described by Maresca and Heald ()andMurray (). To generate low-speed interphase-arrested egg extracts, CSF extract was supplemented with 50 μg/ml cycloheximide and volumes of CaCl 2 solution ( The activity that keeps oocytes arrested in MII was first identified in Rana pipiens eggs and was called cytostatic factor (CSF) by Masui and Markert (). This activity, when injected into a two-cell Xenopus laevis embryo, induces a cell cycle arrest in the M phase.


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Cytostatic factor in Xenopus laevis by Ruth Miriam.* Moses Download PDF EPUB FB2

In Xenopus oocytes, the mos proto-oncogene product is required during meiosis I for the activation of maturation promoting factor (MPF) and the subsequent breakdown of the germinal vesicle (GVBD).

In addition, the mos product has been shown to be a candidate "initiator" of meiotic maturation and is an active component of cytostatic factor (CSF Cited by:   Cytostatic factor (CSF) in unfertilized egg cytoplasm causes metaphase arrest when microinjected into zygotes.

This was originally described in Rana pipiens eggs. In Xenopus laevis, CSF has also been demonstrated, but only when the calcium-chelating agent, EGTA, was injected into the egg the present study, however, CSF was demonstrated in Xenopus eggs when donor egg Cited by: 8.

Meyerhof PG, Masui Y (a) Properties of a cytostatic factor from Xenopus laevis eggs. Dev Biol – PubMed CrossRef Google Scholar Minshull J, Blow JJ, Hunt T () Translation of cyclin mRNA is necessary for extracts of activated Xenopus eggs to enter – Cytostatic factor in Xenopus laevis.

book CrossRef Google ScholarCited by: 3. A role for the anaphase-promoting complex inhibitor Emi2/XErp1, a homolog of early mitotic inhibitor 1, in cytostatic factor arrest of Xenopus eggs.

Proc. Natl Acad. Sci. USA– ()Cited by:   Metaphase II arrest ofXenopusoocyte is characterized by the presence of M-phase-promoting factor (MPF) and of a microtubular spindle, both of which are stable in the presence of protein synthesis studiedin vivothis equilibrium state that is settled during meiotic time of germinal vesicle breakdown (GVBD), cdc2 kinase and MAP kinase activities are by: Xenopus laevis Egg Extract.

Cite this entry as: () Cytostatic Factor (CSF) Egg Extract. In: Dubitzky W., Wolkenhauer O., Cho KH., Yokota H.

(eds) Encyclopedia of. Main Text. Cytostatic factor (CSF) has been studied extensively in Xenopus laevis oocytes, which yield cell-free extracts that faithfully reproduce cell cycle events in vitro.

Hormonal induction causes immature oocytes, which are arrested in meiotic prophase, to undergo maturation: they perform the first meiotic division and then, without an intervening S phase, proceed to metaphase of.

ROLE OF CYCLIC AMP Lag period Intracellular cyclic AMP fluctuations. The endogenous content of cyclic AMP has been investigated in Rana pipiens [3, 4] and Xenopus laevis [5, 6, 7] oocytes and found to be around 1 µM.

The time course for fluctuations in cyclic AMP levels during progesterone-induced maturation shows that a significant decrease of cyclic AMP occurs within 30–60 minutes after.

Cytoplasmic preparation of the egg of the African clawed frog, Xenopus laevis, that is capable of recapitulating many of the biochemical and physiological phenomena of the early embryonic cell phenomena include nuclear envelope formation, DNA replication, DNA condensation, and mitotic spindle formation, all of which can occur after the addition of purified Xenopus sperm chromatin.

Xenopus Egg Extracts Mitotic (Cytostatic Factor) extract. Freshly made, actin-intact cytostatic factor (CSF) egg extract is the starting point all experiments. Detailed protocols for extract preparation were described previously.

Briefly, when laid, unfertilized Xenopus eggs are arrested in metaphase of meiosis-II by CSF. Eggs were de-jellied. Xenopus homolog of the mos protooncogene transforms mammalian fibroblasts and induces maturation of Xenopus oocytes. Proc Natl Acad Sci U S A. Aug; 86 (15)– [PMC free article] Gabrielli BG, Roy LM, Maller JL.

Requirement for Cdk2 in cytostatic factor-mediated metaphase II arrest. Science. Mar 19; ()– However, MPF appearance is blocked by cycloheximide applied before mitosis; and MPF disappearance is blocked by cytostatic factor.

In all these respects, MPF and the inactivating agent seem to be tightly linked to, and perhaps participate in, the cell cycle oscillator previously described for cleaving eggs of Xenopus laevis (Hara, K., P.

Cytostatic factor (CSF) in unfertilized egg cytoplasm causes metaphase arrest when microinjected into zygotes. This was originally described in Rana pipiens eggs In Xenopus laevis, CSF has also been demonstrated. but only when the calcium-chelating agent, EGTA, was injected into the egg cytoplasm.

In the present study, however, CSF was. Unfertilized Xenopus laevis eggs contain a cytostatic factor (CSF) that keeps them in the metaphase stage of the cell cycle. Disruption of the eggs by low-speed centrifugation releases calcium and the eggs cycle into interphase. To probe the function of XCTK2, we have used an in vitro assay for spindle assembly in Xenopus egg extracts.

Addition of antibodies to cytostatic factor-arrested extracts causes a 70% reduction in the percentage of bipolar spindles formed. Proceedings of the National Academy of Sciences - PNAS, ISSN, VolumeIs pp. - Cytostatic Factor and Secondary Cell Cycle Arrest in Meiotic Oocyte Maturation.

At the end of the first meiosis, which is characterized by first polar body extrusion (the appearance of a white spot on the top of the animal hemisphere in the case of Xenopus laevis oocytes), MPF activity declines. After completion of the first meiosis.

Xenopus laevis (African clawed frog) Status. Reviewed-Annotation score: Experimental evidence at Required to prevent anaphase onset in cytostatic factor-arrested oocytes. Inhibits the anaphase-promoting complex/cyclosome (APC/C) ubiquitin ligase and prevents cyclin degradation. Meyerhof PG, Masui Y.

Properties of a cytostatic factor from Xenopus laevis eggs. Dev Biol. Sep; 72 (1)– Watanabe N, Vande Woude GF, Ikawa Y, Sagata N. Specific proteolysis of the c-mos proto-oncogene product by calpain on fertilization of Xenopus eggs.

Nature. Nov 30; ()– Figure 1. Egg membrane microdomain-associated UPIII-Src system in Xenopus laevis.(A) Shown is a schematic diagram for the cell cycle conditions, and the activation/inactivation switching of the MPF (maturation-promoting factor), MAPK (mitogen-activated protein kinase), and UPIII (uroplakin III)-Src system, in the course of oogenesis, oocyte maturation, fertilization, and early embryogenesis in.

A role for the anaphase-promoting complex inhibitor Emi2/XErp1, a homolog of early mitotic inhibitor 1, in cytostatic factor arrest of Xenopus eggs. Proc. Natl Acad. Sci. USA– ().The African clawed frog Xenopus laevis has been instrumental to investigations of both development and cell biology, but the utility of this model organism for genetic and proteomic studies is limited by its long generation time and unsequenced pseudotetraploid s tropicalis, which is a small, faster-breeding relative of X.

laevis, has recently been adopted for research in.Tetsuya Gotoh's 22 research works with citations and reads, including: A Systems Biology Approach Identifies Hidden Regulatory Connections Between the Circadian and Cell-Cycle Checkpoints.